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The study was performed to evaluate the use of equine embryos that were surgically enucleated and matured in vitro and fertilized in vitro to use as a model for developing the maturation technique for equine oocytes in vitro. In experiment 1, thirty equine embryos that were surgically enucleated and matured in vitro were divided into the following 5 groups (n = 10) according to the presence or absence of a corpus luteum (PCL): control (no PCL), fertilized (FPCL), PCL removed (PCLRL) on Day 5, PCLRL on Day 10 and PCLRL on Day 13. After 24 hr of in vitro culture, the cleavage rate and the mean cell number/embryo in the blastocysts were not different (P > 0.05) among groups. However, hatching rate was significantly lower (P 0.05) in the total cell number/blastocyst among groups. In experiment 2, the results were comparable to experiment 1. The total cell number/blastocyst was significantly (P 0.05) in the cleavage rate among groups. These findings showed that equine embryos that were surgically enucleated and matured in vitro were useful as a model for developing the maturation technique for equine oocytes in vitro.Calvin Klein: The Man Behind the Camel
Calvin Klein: The Man Behind the Camel is a 2018 documentary film about American fashion designer Calvin Klein. The documentary, written and directed by Ze Frank, was broadcast on the Netflix streaming service on February 6, 2018.
The documentary tells the story of Calvin Klein, a Jewish-