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We established a specific enzyme-linked immunosorbent assay (ELISA) for simultaneously measuring dopamine and its major metabolite, 3-methoxytyramine, in rat plasma. The assay is based on the competitive ELISA principle. Each drug is immobilized onto microtiter plates in the presence of dopamine. When plasma is added, dopamine competes for binding with immobilized dopamine. Bound and free drugs are detected with specific antibodies and enhanced with an enzyme-conjugated second antibody. The minimum detectable concentrations for dopamine and 3-methoxytyramine were 15.5 and 42.1 pmol/well, respectively, under the present assay conditions. The intra- and inter-assay coefficient of variation for dopamine were 3.1-6.0% (n = 4) and 4.5-7.9% (n = 5), respectively. The intra- and inter-assay coefficient of variation for 3-methoxytyramine were 5.9-7.9% (n = 4) and 5.9-7.6% (n = 5), respectively. Dopamine and 3-methoxytyramine were measured in plasma samples from rats injected with 6-hydroxydopamine, a dopaminergic neurotoxin. Although dopamine concentrations declined to undetectable levels within 15 min of 6-hydroxydopamine injection, the concentration of 3-methoxytyramine reached a plateau immediately after the treatment. This indicates that the major metabolite, 3-methoxytyramine, has a pharmacological effect on the dopaminergic system.The present invention relates to a method and apparatus for removing nail holes and the like from a surface.
The invention has particular application in the case of a residential property

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