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This is a web journal from the Desert Botanical Garden in Phoenix, Arizona. Head to our home page to learn more about our exhibits, educational programs and book store.Detection of insulin receptor autoantibodies in serum of newly diagnosed type I diabetic patients with metabolic decompensation.
Recently, we described insulin receptor autoantibodies (IAA) as serological markers in a subgroup of patients with newly diagnosed type I diabetes mellitus (IDDM) with metabolic decompensation. The aim of the present study was to evaluate the specificity of IAA in patients with recent onset of diabetes. Fifty-nine patients with newly diagnosed IDDM, all of whom presented with severe metabolic decompensation (DR3 or DR4, HLA-DQ heterozygous), were studied and compared with 51 age- and sex-matched healthy controls. HLA-DQ heterozygosity was determined by a genotyping test. The presence of IAA in serum was detected by immunoprecipitation of iodinated receptors followed by autoradiography and immunoprecipitation with an anti-human IAA monoclonal antibody. The specificity of IAA was checked by blocking in a separate series of experiments. There was no significant difference between the two groups regarding age, sex and HLA-DQ genotype distribution. IAA were present in 27 of 59 (45%) patients and in 19 of 51 (37%) controls (p = NS). HLA-DQ genotype analysis showed that no individual haplotype was associated with the presence of IAA. The specificity of IAA was tested by preincubating IAA-positive sera with a dose-dependent amount of specific insulin. The blocking effect was not dose-related. These findings show that serum IAA are not specific markers for diabetes mellitus and, in fact, their presence does not seem to be associated with a particular HLA-DQ genotype.Q:

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